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M199培养液(标准型) 成分表
Media 199 were originally formulated for nutritional studies of chick embryo fibroblasts.
含Earle’s混合盐,1000mg/L D-葡萄糖,100mg/L L-谷氨酰胺,2200mg/L碳酸氢钠。pH值7.0-7.4。
成分
含量
(mg/L)
成分
含量
(mg/L)
无机盐
氨基酸(续前)
CaCl2
200.00
L-Arginine Hydrochloride
70.00
Fe(NO3)3·9H2O
0.70
L-Aspartic Acid
30.00
KCl
400.00
L-Cysteine·HCl·H2O
0.10
MgSO4
97.67
L-Cystine·2HCl
26.00
NaCl
6800.00
L-Glutamic Acid
75.00
NaHCO3
2200.00
L-Glutamine
100.00
NaH2PO4·H2O
140.00
Glycine
50.00
其它组分
L-Histidine ·HCl·H2O
21.88
Adenine Sulfate
10.00
L-Hydroxyproline
10.00
Adenosine 5'-triphosphate
1.00
L-Isoleucine
40.00
Adenosine 5'-phosphate
0.20
L-Leucine
60.00
Cholesterol
0.20
L-Lysine Hydrochloride
70.00
Deoxyribose
0.50
L-Methionine
15.00
D-Glucose
1000.00
L-Phenylalanine
25.00
Glutathione (reduced)
0.05
L-Proline
40.00
Guanine Hydrochloride
0.30
L-Serine
25.00
Hypoxanthine·Na
0.40
L-Threonine
30.00
Phenol Red
20.00
L-Tryptophan
10.00
Ribose
0.50
L-Tyrosine·2Na ·2H20
58.00
Sodium Acetate
50.00
L-Valine
25.00
Thymine
0.30
维生素
Tween 80
20.00
Ascorbic Acid
0.05
Uracil
0.30
Alpha-Tocopherol Phosphate
0.01
Xanthine·Na
0.34
Biotin
0.01
氨基酸
Calciferol (Vitamin D2)
0.10
L-Alanine
25.00
D-Calcium Pantothenate
0.01
成分
含量
(mg/L)
成分
含量
(mg/L)
维生素(续前)
Choline Chloride
0.50
Folic Acid
0.01
i-Inositol
0.05
Menadione (Vitamin K3)
0.01
Niacin
0.025
Niacinamide
0.025
Para-aminobenzoic acid
0.05
Pyridoxal Hydrochloride
0.025
Pyridoxine Hydrochloride
0.025
Riboflavin
0.01
Thiamine Hydrochloride
0.01
Vitamin A (acetate)
0.10