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α-MEM－培养液 成分表
Alpha modification of the MEM medium includes the addition of the non-essential amino acids, sodium pyruvate, lipoic acid, ascorbic acid, biotin and vitamin B12.One useful application for alpha MEM is as a selection media. Dihydrofolate Reductase (DHFR) is an enzyme which is necessary for purine synthesis. In the absence of exogenous purines, this enzyme is required for growth. Alpha MEM minus does not contain the ribonucleosides or the deoxyribonucleosides, and will not support the growth of cells that do not contain the gene to produce DHFR.
含核糖核酸和脱氧核糖核酸，1000mg/L D-葡萄糖，292 mg/L L-谷氨酰胺，110mg/L丙酮酸钠，2200mg/L碳酸氢钠。 pH值7.0-7.4。
成分
含量
(mg/L)成分
含量
(mg/L)无机盐:
氨基酸（续前）
CaCl2 (anhyd.)
200.00
L-Histidine
31.00
KCl
400.00
L-Isoleucine
52.40
MgSO4 (anhyd.)
97.67
L-Leucine
52.40
NaCl
6800.00
L-Lysine
58.00
NaHCO3
2200.00
L-Methionine
15.00
NaH2PO4·H2O
140.00
L-Phenylalanine
32.00
其它组分:
L-Proline
40.00
D-Glucose
1000.00
L-Serine
25.00
Lipoic Acid
0.20
L-Threonine
48.00
Phenol Red
10.00
L-Tryptophan
10.00
Sodium Pyruvate
110.00
L-Tyrosine (disodium salt)
52.00
氨基酸:
L-Valine
46.00
L-Alanine
25.00
维生素
L-Arginine
105.00
L-Ascorbic Acid
50.00
L-Asparagine·H2O
50.00
Biotin
0.10
L-Aspartic Acid
30.00
D-Ca Pantothenate
1.00
L-Cystine·2HCl
24.00
Choline Chloride
1.00
L-Cysteine·HCl·H2O
100.00
Folic Acid
1.00
L-Glutamic Acid
75.00
i-Inositol
2.00
L-Glutamine
292.00
Niacinamide
1.00
Glycine
50.00
成分
含量
(mg/L)
成分
含量
(mg/L)
维生素（续前
Pyridoxal HCl
1.00
Riboflavin
0.10
Thiamine HCl
1.00
Vitamin B12
1.36
核糖核酸
Adenosine
10.00
Cytidine
10.00
Guanosine
10.00
Uridine
10.00
脱氧核糖核酸
2' Deoxyadenosine
10.00
2' Deoxycytidine HCl
11.00
2' Deoxyguanosine
10.00
Thymidine
10.00