产品中心
- 产品简介
- 使用说明
- 相关产品
- 参考文献
- 购买咨询
F12培养液(Ham) 成分表
F-12 Nutrient Mixture was originally formulated for single cell plating of near-diploid Chinese Hamster Ovary (CHO) cells.
含146mg/L L-谷氨酰胺,1802 mg/L D-葡萄糖, 110mg/L丙酮酸钠,1176mg/L碳酸氢钠,pH值7.2-7.4。
成分
含量
成分
含量
(mg/L)
氨基酸(续前)
(mg/L)
无机盐:
L-Glutamine
146.00
CaCl2
33.22
Glycine
7.50
CuSO4·5H2O
0.0025
L-Histidine·HCl·H2O
21.00
FeSO4·7H2O
0.834
L-Isoleucine
4.00
KCl
223.60
L-Leucine
13.10
MgCl2
57.22
L-Lysine Hydrochloride
36.50
NaCl
7599.00
L-Methionine
4.50
NaHCO3
1176.00
L-Phenylalanine
5.00
Na2HPO4
142.00
L-Proline
34.50
ZnSO4·7H2O
0.863
L-Serine
10.50
其它组分:
L-Threonine
11.90
D-Glucose
1802.00
L-Tryptophan
2.04
Hypoxanthine·Na
4.77
L-Tyrosine·2Na·2H2O
7.81
Linoleic Acid
0.084
L-Valine
11.70
Lipoic Acid
0.21
维生素:
Phenol Red
1.20
Biotin
0.0073
Putrescine·2HCl
0.161
D-Calcium Pantothenate
0.50
Sodium Pyruvate
110.00
Choline Chloride
14.00
Thymidine
0.70
Folic Acid
1.30
氨基酸
i-Inositol
18.00
L-Alanine
8.90
Niacinamide
0.036
L-Arginine Hydrochloride
211.00
Pyridoxine Hydrochloride
0.06
L-Asparagine·H2O
15.01
Riboflavin
0.037
L-Aspartic Acid
13.30
Thiamine Hydrochloride
0.30
L-Cysteine·HCl·H2O
35.12
Vitamin B12
1.40
L-Glutamic Acid
14.70