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MEM－培养液 成分表
MEM (Mimimum Essential Medium) was developed when Basal Media Eagle was not sufficient to support Hela cells. It was discovered that with certain media supplements, BME could be made to grow a wider variety of cells. MEM incorporates these modifications, such as higher concentrations of amino acids into its formulation.
含Earle's混合盐, 1000 mg/L D-葡萄糖,292mg/L谷氨酰胺, 2200mg/L碳酸氢钠; 不含非必需氨基酸。 pH值7.0-7.4。
成 分
含 量
(mg/L)
成 分
含 量
(mg/L)
无机盐:
氨基酸（续前）
CaCl2 (anhyd.)
200.00
L-Tyrosine (disodium salt)
52.00
KCl
400.00
L-Valine
46.00
MgSO4 (anhyd.)
97.67
维生素
NaCl
6800.00
D-Ca Pantothenate
1.00
NaHCO3
2200.00
Choline Chloride
1.00
NaH2PO4·H2O
140.00
Folic Acid
1.00
其它组分
i-Inositol
2.00
D-Glucose
1000.00
Niacinamide
1.00
Phenol Red
10.00
Pyridoxal HCl
1.00
氨基酸:
Riboflavin
0.10
L-Arginine·HCl
126.64
Thiamine HCl
1.00
L-Cystine·2HCl
31.00
L-Glutamine
292.00
L-Histidine·HCl·H2O
42.00
L-Isoleucine
52.00
L-Leucine
52.00
L-Lysine·HCl
72.50
L-Methionine
15.00
L-Phenylalanine
32.00
L-Threonine
48.00
L-Tryptophan
10.00