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α-MEM－培养液 成分表
Alpha modification of the MEM medium includes the addition of the non-essential amino acids, sodium pyruvate, lipoic acid, ascorbic acid, biotin and vitamin B12.One useful application for alpha MEM is as a selection media. Dihydrofolate Reductase (DHFR) is an enzyme which is necessary for purine synthesis. In the absence of exogenous purines, this enzyme is required for growth. Alpha MEM minus does not contain the ribonucleosides or the deoxyribonucleosides, and will not support the growth of cells that do not contain the gene to produce DHFR.
不含核糖核酸和脱氧核糖核酸，含1000mg/L D-葡萄糖，292 mg/L L-谷氨酰胺，110mg/L丙酮酸钠，2200mg/L碳酸氢钠。 pH值7.0-7.4。
成分
含量
(mg/L)
成分
含量
(mg/L)
无机盐:
氨基酸（续前）
CaCl2
200.00
L-Leucine
52.40
KCl
400.00
L-Lysine
58.00
MgSO4
97.67
L-Methionine
15.00
NaCl
6800.00
L-Phenylalanine
32.00
NaHCO3
2200.00
L-Proline
40.00
NaH2PO4·H2O
140.00
L-Serine
25.00
其它组分:
L-Threonine
48.00
D-Glucose
1000.00
L-Tryptophan
10.00
Lipoic Acid
0.20
L-Tyrosine (disodium salt)
52.00
Phenol Red
10.00
L-Valine
46.00
Sodium Pyruvate
110.00
维生素
氨基酸:
L-Ascorbic Acid
50.00
L-Alanine
25.00
Biotin
0.10
L-Arginine
105.00
D-Ca Pantothenate
1.00
L-Asparagine·H2O
50.00
Choline Chloride
1.00
L-Aspartic Acid
30.00
Folic Acid
1.00
L-Cystine·2HCl
24.00
i-Inositol
2.00
L-Cysteine·HCl·H2O
100.00
Niacinamide
1.00
L-Glutamic Acid
75.00
Pyridoxal HCl
1.00
L-Glutamine
292.00
Riboflavin
0.10
Glycine
50.00
Thiamine HCl
1.00
L-Histidine
31.00
Vitamin B12
1.36
L-Isoleucine
52.40