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CI82
Recombinant Human Methylmalonyl-CoA epimerase/MCEE
10ug
560
504
现货
国产
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CI82
Recombinant Human Methylmalonyl-CoA epimerase/MCEE
50ug
1680
1512
现货
国产
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CI82
Recombinant Human Methylmalonyl-CoA epimerase/MCEE
500ug
12320
11088
现货
国产
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CI82
Recombinant Human Methylmalonyl-CoA epimerase/MCEE
1mg
17600
15840
现货
国产
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Catalog# CI82 Source Human cells Description Recombinant Human Methylmalonyl-CoA epimerase is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Gln37-Ala176) of Human MCEE fused with a polyhistidine tag at the C-terminus. Names Methylmalonyl-CoA epimerase, mitochondrial,DL-methylmalonyl-CoA racemase Accession # Q96PE7 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,1mM DTT,10%Glycerol,pH7.5 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence QVTGSVWNLGRLNHVAIAVPDLEKAAAFYKNILGAQVSEAVPLPEHGVSVVFVNLGNTKMELLHP LGLDSPIAGFLQKNKAGGMHHICIEVDNINAAVMDLKKKKIRSLSEEVKIGAHGKPVIFLHPKDC GGVLVELEQALDHHHHHHBackground Methylmalonyl-CoA epimerase, mitochondrial（MCEE）is an enzyme which belongs to the glyoxalase I family. It converts (S)-methylmalonyl-CoA to the (R) form, catalyses the following chemical reaction: (R)-methylmalonyl-CoA (S)-methylmalonyl-CoA. It plays an important role in the catabolism of fatty acids with odd-length carbon chains. This protein deficiency is an autosomal recessive inborn error of AA metabolism, involving valine, threonine, isoleucine and methionine. This organic aciduria can appear in the neonatal period with life-threatening metabolic acidosis, hyperammonemia, feeding difficulties, pancytopenia and coma.