产品中心
-
产品货号
产品名称
规格
销售价
会员价
货期
产地
订购
-
CI62
Recombinant Human PAPSS1/Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
10ug
1200
1080
现货
国产
-
CI62
Recombinant Human PAPSS1/Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
50ug
3520
3168
现货
国产
-
CI62
Recombinant Human PAPSS1/Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
500ug
12320
11088
现货
国产
-
CI62
Recombinant Human PAPSS1/Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1
1mg
17600
15840
现货
国产
- 产品简介
- 使用说明
- 相关产品
- 参考文献
- 购买咨询
Catalog# CI62 Source Human cells Description Recombinant Human PAPSS1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Ala624) of Human PAPSS1 fused with a polyhistidine tag at the C-terminus. Names Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1,PAPS synthase 1,PAPSS 1,Sulfurylase kinase 1,SK 1,ATPSK1, PAPSS Accession # O43252 Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl buffer (pH 8.0), 100mM NaCl and 20% glycerol Shipping The product is shipped on dry ice/ice packs. Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence MEIPGSLCKKVKLSNNAQNWGMQRATNVTYQAHHVSRNKRGQVVGTRGGFRGCTVWLTGLSGAGK TTVSMALEEYLVCHGIPCYTLDGDNIRQGLNKNLGFSPEDREENVRRIAEVAKLFADAGLVCITS FISPYTQDRNNARQIHEGASLPFFEVFVDAPLHVCEQRDVKGLYKKARAGEIKGFTGIDSEYEKP EAPELVLKTDSCDVNDCVQQVVELLQERDIVPVDASYEVKELYVPENKLHLAKTDAETLPALKIN KVDMQWVQVLAEGWATPLNGFMREREYLQCLHFDCLLDGGVINLSVPIVLTATHEDKERLDGCTA FALMYEGRRVAILRNPEFFEHRKEERCARQWGTTCKNHPYIKMVMEQGDWLIGGDLQVLDRVYWN DGLDQYRLTPTELKQKFKDMNADAVFAFQLRNPVHNGHALLMQDTHKQLLERGYRRPVLLLHPLG GWTKDDDVPLMWRMKQHAAVLEEGVLNPETTVVAIFPSPMMYAGPTEVQWHCRARMVAGANFYIV GRDPAGMPHPETGKDLYEPSHGAKVLTMAPGLITLEIVPFRVAAYNKKKKRMDYYDSEHHEDFEF ISGTRMRKLAREGQKPPEGFMAPKAWTVLTEYYKSLEKAVDHHHHHHBackground PAPSS1 is a bifunctional enzyme with both ATP sulfurylase and APS kinase activity. In the N-terminal section, it belongs to the APS kinase family; while the C-terminal section belongs to the sulfate adenylyltransferase family. PAPSS1 can be inhibited by chlorate, and is expressed in many tissues, such as high endothelial venules (HEV) cells and in cartilage. PAPSS1 mediates two steps in the sulfate activation pathway. The first step is the transfer of a sulfate group to ATP to yield adenosine 5'-phosphosulfate (APS), and the second step is the transfer of a phosphate group from ATP to APS yielding 3'-phosphoadenylylsulfate. In mammals, PAPS is the sole source of sulfate; APS appears to be only an intermediate in the sulfate-activation pathway. PAPSS1 also involved in the biosynthesis of sulfated L-selectin ligands in endothelial cells.