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CJ35
Recombinant Human PIP4K2A/Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
10ug
1200
1080
现货
国产
-
CJ35
Recombinant Human PIP4K2A/Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
50ug
3520
3168
现货
国产
-
CJ35
Recombinant Human PIP4K2A/Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
500ug
14800
13320
现货
国产
-
CJ35
Recombinant Human PIP4K2A/Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha
1mg
21120
19008
现货
国产
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Catalog# CJ35 Source Human cells Description Recombinant Human PIP4K2A is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Thr406) of Human PIP4K2A fused with a polyhistidine tag at the C-terminus. Names 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha,Diphosphoinositide kinase 2-alpha,PIP5KIII,Phosphatidylinositol 5-phosphate 4-kinase type II alpha,PtdIns(4)P-5-kinase B isoform,PtdIns(4)P-5-kinase C isoform,PtdIns(5)P-4-kinase isoform 2-alpha Accession # P48426 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence MATPGNLGSSVLASKTKTKKKHFVAQKVKLFRASDPLLSVLMWGVNHSINELSHVQIPVMLMPDD FKAYSKIKVDNHLFNKENMPSHFKFKEYCPMVFRNLRERFGIDDQDFQNSLTRSAPLPNDSQARS GARFHTSYDKRYIIKTITSEDVAEMHNILKKYHQYIVECHGITLLPQFLGMYRLNVDGVEIYVIV TRNVFSHRLSVYRKYDLKGSTVAREASDKEKAKELPTLKDNDFINEGQKIYIDDNNKKVFLEKLK KDVEFLAQLKLMDYSLLVGIHDVERAEQEEVECEENDGEEEGESDGTHPVGTPPDSPGNTLNSSP PLAPGEFDPNIDVYGIKCHENSPRKEVYFMAIIDILTHYDAKKKAAHAAKTVKHGAGAEISTVNP EQYSKRFLDFIGHILTVDHHHHHHBackground Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha (PIP4K2A) is a member of the phosphatidylinositol-4-phosphate 5-kinase family. It contains 1 PIPK domain and is expressed ubiquitously, with high levels in the brain. It catalyzes the phosphorylation of phosphatidylinositol 5-phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). It may exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. It may regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation, negatively regulate insulin-stimulated glucose uptake by lowering the levels of PtdIns5P. It also involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size.