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CJ15
Recombinant Human PLA2G1B/Phospholipase A2/PLA2/PLA2A/PPLA2
10ug
1200
1080
现货
国产
-
CJ15
Recombinant Human PLA2G1B/Phospholipase A2/PLA2/PLA2A/PPLA2
50ug
3520
3168
现货
国产
-
CJ15
Recombinant Human PLA2G1B/Phospholipase A2/PLA2/PLA2A/PPLA2
500ug
14800
13320
现货
国产
-
CJ15
Recombinant Human PLA2G1B/Phospholipase A2/PLA2/PLA2A/PPLA2
1mg
21120
19008
现货
国产
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Catalog# CJ15 Source Human cells Description Recombinant Human PLA2G1B is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ala23-Ser148) of Human PLA2G1B fused with a polyhistidine tag at the C-terminus. Names PLA2G1B/Phospholipase A2/Group IB phospholipase A2/PLA2/PLA2A/PPLA2 Accession # P04054 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM TrisHCl,150mM NaCl,10% Glycerol,pH8.0 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 5X PBS.
Please aliquot the reconstituted solutionStorage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 7 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence AVWQFRKMIKCVIPGSDPFLEYNNYGCYCGLGGSGTPVDELDKCCQTHDNCYDQAKKLDSCKFLL DNPYTHTYSYSCSGSAITCSSKNKECEAFICNCDRNAAICFSKAPYNKAHKNLDTKKYCQSVDHH HHHHBackground Phospholipase A2(PLA2G1B) is a secreted protein which belongs to the phospholipase A2 family. It catalyzes the release of fatty acids from glycero-3-phosphocholines. It catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. This releases glycerophospholipids and arachidonic acid that serve as the precursors of signal molecules. Sequences of pancreatic PLA2G1B enzymes from a variety of mammals have been reported. One striking feature of these enzymes is their close homology to venom phospholipases of snakes. Mice lacking in PLA2G1B are resistant to obesity and diabetes induced by feeding a diabetogenic high-fat/high-carbohydrate diet. Oral supplementation of a diabetogenic diet with the PLA2G1B inhibitor methyl indoxam effectively suppresses diet-induced obesity and diabetes. PLA2G1B inhibition may be a potentially effective oral therapeutic option for treatment of obesity and diabetes.