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CH81
Recombinant Human Pyruvate kinase PKM/PKM2
10ug
840
756
现货
国产
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CH81
Recombinant Human Pyruvate kinase PKM/PKM2
50ug
2520
2268
现货
国产
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CH81
Recombinant Human Pyruvate kinase PKM/PKM2
500ug
12320
11088
现货
国产
-
CH81
Recombinant Human Pyruvate kinase PKM/PKM2
1mg
17600
15840
现货
国产
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Catalog# CH81 Source E.coli Description Recombinant Human PKM2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Pro531) of Human PKM2 fused with a 6His tag at the C-terminus. Names Cytosolic thyroid hormone-binding protein,Opa-interacting protein 3,Pyruvate kinase 2/3,Pyruvate kinase muscle isozyme,Thyroid hormone-binding protein 1,Tumor M2-PK,p58,CTHBP,OIP3,PK2,PK3,TCB,THBP1 Accession # P14618 Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris,1mM DTT,0.1M NaCl,pH8.0 Shipping The product is shipped on dry ice/ice packs. Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence MSKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSPPITARNTGIICTIGPASRSVETLKEMI KSGMNVARLNFSHGTHEYHAETIKNVRTATESFASDPILYRPVAVALDTKGPEIRTGLIKGSGTA EVELKKGATLKITLDNAYMEKCDENILWLDYKNICKVVEVGSKIYVDDGLISLQVKQKGADFLVT EVENGGSLGSKKGVNLPGAAVDLPAVSEKDIQDLKFGVEQDVDMVFASFIRKASDVHEVRKVLGE KGKNIKIISKIENHEGVRRFDEILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVI CATQMLESMIKKPRPTRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIY HLQLFEELRRLAPITSDPTEATAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTR NPQTARQAHLYRGIFPVLCKDPVQEAWAEDVDLRVNFAMNVGKARGFFKKGDVVIVLTGWRPGSG FTNTMRVVPVPLEHHHHHHBackground Pyruvate kinase PKM is an isoenzyme of the glycolytic enzyme pyruvate kinase. Depending upon the different metabolic functions of the tissues, different isoenzymes of pyruvate kinase are expressed. The pyruvate kinase isoenzyme type M2（PKM2) is expressed in some differentiated tissues, such as lung, fat tissue, retina, and pancreatic islets, as well as in all cells with a high rate of nucleic acid synthesis, such as normal proliferating cells, embryonic cells, and especially tumor cells. As a glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. It stimulates POU5F1-mediated transcriptional activation and Plays a general role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.