产品中心
-
产品货号
产品名称
规格
销售价
会员价
货期
产地
订购
-
CH56
Recombinant Human Ubiquitin-conjugating enzyme E2 D1/UBE2D1/UBCH5
10ug
400
360
现货
国产
-
CH56
Recombinant Human Ubiquitin-conjugating enzyme E2 D1/UBE2D1/UBCH5
50ug
1120
1008
现货
国产
-
CH56
Recombinant Human Ubiquitin-conjugating enzyme E2 D1/UBE2D1/UBCH5
500ug
3480
3132
现货
国产
-
CH56
Recombinant Human Ubiquitin-conjugating enzyme E2 D1/UBE2D1/UBCH5
1mg
4960
4464
现货
国产
- 产品简介
- 使用说明
- 相关产品
- 参考文献
- 购买咨询
Catalog# CH56 Source E.coli Description Recombinant Human Ubiquitin-conjugating enzyme E2 D1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Met147) of Human UBE2D1 fused with a GST tag at the N-terminus. Names Ubiquitin-conjugating enzyme E2 D1,Stimulator of Fe transport,SFT,UBC4/5 homolog,UbcH5,Ubiquitin carrier protein D1,Ubiquitin-conjugating enzyme E2(17)KB 1,Ubiquitin-conjugating enzyme E2-17 kDa 1,Ubiquitin-protein ligase D1,SFT, UBC5A, UBCH5, UBCH5A Accession # P51668 Formulation Lyophilized from a 0.2 μm filtered solution of 50mM HEPES，150mM NaCl，2mM DTT,10% Glycerin pH7.5 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKL TQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEML KMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKS SKYIAWPLQGWQATFGGGDHPPKSDLVPRGSMALKRIQKELSDLQRDPPAHCSAGPVGDDLFHWQ ATIMGPPDSAYQGGVFFLTVHFPTDYPFKPPKIAFTTKIYHPNINSNGSICLDILRSQWSPALTV SKVLLSICSLLCDPNPDDPLVPDIAQIYKSDKEKYNRHAREWTQKYAM*Background Ubiquitin-conjugating enzyme E2 D1（UBE2D1）belongs to the ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This enzyme is closely related to a stimulator of iron transport (SFT), and is up-regulated in hereditary hemochromatosis. It also functions in the ubiquitination of the tumor-suppressor protein p53 and the hypoxia-inducible transcription factor HIF1alpha by interacting with the E1 ubiquitin-activating enzyme and the E3 ubiquitin-protein ligases.