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C554
Recombinant Human Alkaline Phosphatase/ALPL
10ug
1200
1080
现货
国产
-
C554
Recombinant Human Alkaline Phosphatase/ALPL
50ug
3520
3168
现货
国产
-
C554
Recombinant Human Alkaline Phosphatase/ALPL
500ug
12320
11088
现货
国产
-
C554
Recombinant Human Alkaline Phosphatase/ALPL
1mg
17600
15840
现货
国产
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Catalog# C554 Source HEK293 Description Recombinant Human Alkaline Phosphatase, Tissue-Nonspecific Isozyme/ALPL is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Leu18-Ser502) of Human ALPL fused with a polyhistidine tag at the C-terminus. Names Alkaline Phosphatase, Tissue-Nonspecific Isozyme, AP-TNAP, TNSALP, Alkaline Phosphatase Liver/Bone/Kidney Isozyme, ALPL Accession # P05186 Formulation Supplied as a 0.2 μm filtered solution of 20mM HEPES, 150mM NaCl, 2mM MgSO4, 0.1mM ZnCl2, pH 7.5 Shipping The product is shipped on dry ice/ice packs. Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.Biological Activity Special Activity: 66 U/mg based on useing p-nitrophenyl phosphate (pNPP) as a phosphatase substrate which turns yellow (λmax= 405 nm) when dephosphorylated by ALP. At pH10.4, 37⁰C, 1 Unit is defined as cleaving 1.0 µmol p-nitrophenyl phosphate (pNPP) per minute. Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence LVPEKEKDPKYWRDQAQETLKYALELQKLNTNVAKNVIMFLGDGMGVSTVTAARILKGQLHHNPG EETRLEMDKFPFVALSKTYNTNAQVPDSAGTATAYLCGVKANEGTVGVSAATERSRCNTTQGNEV TSILRWAKDAGKSVGIVTTTRVNHATPSAAYAHSADRDWYSDNEMPPEALSQGCKDIAYQLMHNI RDIDVIMGGGRKYMYPKNKTDVEYESDEKARGTRLDGLDLVDTWKSFKPRYKHSHFIWNRTELLT LDPHNVDYLLGLFEPGDMQYELNRNNVTDPSLSEMVVVAIQILRKNPKGFFLLVEGGRIDHGHHE GKAKQALHEAVEMDRAIGQAGSLTSSEDTLTVVTADHSHVFTFGGYTPRGNSIFGLAPMLSDTDK KPFTAILYGNGPGYKVVGGERENVSMVDYAHNNYQAQSAVPLRHETHGGEDVAVFSKGPMAHLLH GVHEQNYVPHVMAYAACIGANLGHCAPASSVDVHHHHHHBackground Alkaline Phosphatase, Tissue-Nonspecific Isozyme (ALPL) is a cell membrane protein which belongs to the alkaline phosphatase family. There are at least four distinct but related alkaline phosphatases in humans: intestinal AP (IAP), placental AP(PLAP), germ cell AP (GCAP) and their genes are clustered on chromosome 2, tissue-nonspecific isozyme (TNAP) which gene is located on chromosome 1. Alkaline phosphatases (APs) are dimeric enzymes, it catalyze the hydrolysis of phosphomonoesters with release of inorganic phosphate. The native ALPL is a glycosylated homodimer attached to the membrane through a GPI-anchor. This isozyme may play a role in skeletal mineralization. Mutations in ALPL gene have been linked directly to different forms of hypophosphatasia,characterized by poorly mineralized cartilage and bones, and this disorder can vary depending on the specific mutation since this determines age of onset and severity of symptoms.