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C962
Recombinant Human BCMO1/β-carotene dioxygenase 1
10ug
1200
1080
现货
国产
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C962
Recombinant Human BCMO1/β-carotene dioxygenase 1
50ug
3520
3168
现货
国产
-
C962
Recombinant Human BCMO1/β-carotene dioxygenase 1
500ug
12320
11088
现货
国产
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C962
Recombinant Human BCMO1/β-carotene dioxygenase 1
1mg
17600
15840
现货
国产
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Catalog# C962 Source Human Cells Description Recombinant Human β,β-Carotene 15,15'-Monooxygenase/BCMO1 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Thr547) of Human BCMO1 fused with a polyhistidine tag at the C-terminus. Names Beta,Beta-Carotene 15,15'-Monooxygenase, Beta-Carotene Dioxygenase 1, BCMO1, BCDO, BCDO1 Accession # Q9HAY6 Formulation Lyophilized from a 0.2 μm filtered solution of 50mM Tris HCl,10mM reduced Glutathione,pH 8.0 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence MDIIFGRNRKEQLEPVRAKVTGKIPAWLQGTLLRNGPGMHTVGESRYNHWFDGLALLHSFTIRDG EVYYRSKYLRSDTYNTNIEANRIVVSEFGTMAYPDPCKNIFSKAFSYLSHTIPDFTDNCLINIMK CGEDFYATSETNYIRKINPQTLETLEKVDYRKYVAVNLATSHPHYDEAGNVLNMGTSIVEKGKTK YVIFKIPATVPEGKKQGKSPWKHTEVFCSIPSRSLLSPSYYHSFGVTENYVIFLEQPFRLDILKM ATAYIRSMSWASCLAFHREEKTYIHIIDQRTRQPVQTKFYTDAMVVFHHVNAYEEDGCIVFDVIA YEDNSLYQLFYLANLNQDFKENSRLTSVPTLRRFAVPLHVDKNAEVGTNLIKVASTTATALKEED GQVYCQPEFLYEGLELPRVNYAHNGKQYRYVFATGVQWSPIPTKIIKYDILTKSSLKWREDDCWP AEPLFVPAPGAKDEDDGVILSAIVSTDPQKLPFLLILDAKSFTELARASVDVDMHMDLHGLFITD MDWDTKKQAASEEQRDRASDCHGAPLTVDHHHHHHBackground β,β-Carotene 15,15'-Monooxygenase (BCMO1) is a member of the carotenoid oxygenase family. BCMO1 is highly expressed in retinal pigment epithelium, it is also expressed in the kidney, testis, liver, brain, small intestine, and colon. BCMO1 is a key enzyme in β-carotene metabolism to vitamin A. BCMO1 catalyzes the oxidative cleavage of β,β-carotene into two retinal molecules. The reaction proceeds in three stages, epoxidation of the 15,15'-double bond, hydration of the double bond leading to ring opening, and oxidative cleavage of the diol formed. Defects in BCMO1 are the cause of autosomal dominant hypercarotenemia and vitamin A deficiency.