产品中心
-
产品货号
产品名称
规格
销售价
会员价
货期
产地
订购
-
C562
Recombinant Human Biotinidase/Biotinase/BTD
10ug
1200
1080
现货
国产
-
C562
Recombinant Human Biotinidase/Biotinase/BTD
50ug
3520
3168
现货
国产
-
C562
Recombinant Human Biotinidase/Biotinase/BTD
500ug
12320
11088
现货
国产
-
C562
Recombinant Human Biotinidase/Biotinase/BTD
1mg
17600
15840
现货
国产
- 产品简介
- 使用说明
- 相关产品
- 参考文献
- 购买咨询
Catalog# C562 Source HEK293 Description Recombinant Human Biotinidase/BTD is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ala42-Asp543) of Human BTD fused with a polyhistidine tag at the C-terminus. Names Biotinidase, Biotinase, BTD Accession # P43251 Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5 Shipping The product is shipped on dry ice/ice packs. Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence AHTGEESVADHHEAEYYVAAVYEHPSILSLNPLALISRQEALELMNQNLDIYEQQVMTAAQKDVQ IIVFPEDGIHGFNFTRTSIYPFLDFMPSPQVVRWNPCLEPHRFNDTEVLQRLSCMAIRGDMFLVA NLGTKEPCHSSDPRCPKDGRYQFNTNVVFSNNGTLVDRYRKHNLYFEAAFDVPLKVDLITFDTPF AGRFGIFTCFDILFFDPAIRVLRDYKVKHVVYPTAWMNQLPLLAAIEIQKAFAVAFGINVLAANV HHPVLGMTGSGIHTPLESFWYHDMENPKSHLIIAQVAKNPVGLIGAENATGETDPSHSKFLKILS GDPYCEKDAQEVHCDEATKWNVNAPPTFHSEMMYDNFTLVPVWGKEGYLHVCSNGLCCYLLYERP TLSKELYALGVFDGLHTVHGTYYIQVCALVRCGGLGFDTCGQEITEATGIFEFHLWGNFSTSYIF PLFLTSGMTLEVPDQLGWENDHYFLRKSRLSSGLVTAALYGRLYERDVDHHHHHHBackground Biotinidase is a secreted enzyme that belongs to the CN hydrolase family of BTD/VNN subfamily. Biotinidase Contains 1 CN hydrolase domain. Biotinidase allows the body to use and to recycle the B vitamin biotin, sometimes called vitamin H. Biotinidase extracts biotin from food because the body needs biotin in its free, unattached form. This enzyme also recycles biotin from enzymes in the body that use it as a helper component in order to function. Biotinidase catalyzes release of biotin from biocytin, the product of biotin-dependent carboxylases degradation. These enzymes, known as carboxylases, are important in the processing of fats, carbohydrates, and proteins. Biotin is attached to these carboxylase enzymes through lysine, forming a complex called biocytin. Biotinidase removes biotin from biocytin and makes it available to be reused by other enzymes. Defects in Biotinidase causes biotinidase deficiency, which is a multiple carboxylase deficiency, characterized by seizures, hypotonia, skin rash, alopecia, ataxia, hearing loss, and optic atrophy.