| Catalog# |
C012 |
| Source |
E.coli |
| Description |
Recombinant Human Bone Morphogenetic Protein 2/BMP-2 is produced by our E. coli expression system. The target protein is expressed with sequence (Gln283-Arg396) of Human BMP-2. |
| Names |
Bone Morphogenetic Protein 2, BMP-2, Bone Morphogenetic Protein 2A, BMP-2A, BMP2, BMP2A |
| Accession # |
P12643 |
| Formulation |
Lyophilized from a 0.2 μm filtered solution of 50mM HAc |
| Shipping |
The product is shipped at ambient temperature. |
| Reconstitution |
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage |
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Biological Activity |
Recombinant BMP-2 is fully biologically active when compared to standards.
ED50 is less than 50 ng/ml as determined by the cytolysis of MC3T3-E1 cells.
Specific Activity of 2.0 x 104 IU/mg |
| Purity |
Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. |
| Endotoxin |
Less than 0.1 ng/µg (1 IEU/µg). |
| Amino Acid Sequence |
MQAKHKQRKRLKSSCKRHPLYVDFSDVGWNDWIVAPPGYHAFYCHGECPFPLADHLNSTNHAIVQ TLVNSVNSKIPKACCVPTELSAISMLYLDENEKVVLKNYQDMVVEGCGCR
|
| Background |
Bone Morphogenetic Protein-2 (BMP-2) is one of the bone-growth regulatory factors that belong to the transforming growth factor-beta (TGF-beta) superfamily of proteins. BMPs are synthesized as large precursor molecules, which are cleaved by proteolytic enzymes. The active form of BMP-2 can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins. |
| References |
Wu T,et al.miR-30 Family Members Negatively Regulate Osteoblast Differentiation
PMID:22253433
http://www.ncbi.nlm.nih.gov/pubmed/22253433 |
