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C075
Recombinant Human C5a/Complement Component C5
10ug
1080
972
现货
国产
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C075
Recombinant Human C5a/Complement Component C5
50ug
3200
2880
现货
国产
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C075
Recombinant Human C5a/Complement Component C5
500ug
12320
11088
现货
国产
-
C075
Recombinant Human C5a/Complement Component C5
1mg
17600
15840
现货
国产
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Catalog# C075 Source E.coli Description Recombinant Mouse Complement C5a produced in E. coli is a single non-glycosylated polypeptide chain containing 78 amino acids (N679-R755) with a molecular mass of 9 kD. Names Complement C5, Hemolytic Complement, C5, Hc Accession # P06684 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 350mM NaCl, pH 7.5 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by reducing RP-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence MNLHLLRQKIEEQAAKYKHSVPKKCCYDGARVNFYETCEERVARVTIGPLCIRAFNECCTIANKI RKESPHKPVQLGRBackground Mouse Complement C5 (C5a) is a glycoprotein that belongs to a family of structurally and functionally related proteins known as anaphylatoxins. C5a is a 77 amino acid peptide that is created by the C5a convertase proteolytic cleavage of C5 αchain in the classical and alternative complement pathway (C4b2a3b, C3bBb3b). Mouse C5a has fourαhelices, plus three intra-chain disulfide bonds that form a triple loop structure. C5a functions via G-protein coupled receptor (GPCR) (C5aR/CD88). C5a is a potent chemoattractant and anaphylatoxin that acts on all classes of leukocytes and on many other cell types including endothelial, smooth muscle, kidney, liver, and neural cells. It mediates IL-8 release from bronchial epithelial cells. It also triggers an oxidative burst in macrophages and neutrophils, causing release of histamine in basophils and mast cells. C5a anaphylatoxin activity on hepatocytes results indirectly from interaction with nonparenchymal cell via prostanoid secretion. Mouse C5a shares 60% and 82% sequence identity to human and rat C5a, respectively.