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CI32

Recombinant Human CHRNB3/Neuronal acetylcholine receptor subunit beta-3

10ug

480

432

现货

国产

CI32

Recombinant Human CHRNB3/Neuronal acetylcholine receptor subunit beta-3

50ug

1440

1296

现货

国产

CI32

Recombinant Human CHRNB3/Neuronal acetylcholine receptor subunit beta-3

500ug

12320

11088

现货

国产

CI32

Recombinant Human CHRNB3/Neuronal acetylcholine receptor subunit beta-3

1mg

17600

15840

现货

国产

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  • Catalog# CI32
    Source Human Cells
    Description Recombinant Human Neuronal acetylcholine receptor subunit beta-3 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Ile25-Leu232) of Human CHRNB3 fused with a 6His tag at the C-terminus.
    Names Neuronal acetylcholine receptor subunit beta-3
    Accession # Q05901
    Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4
    Shipping The product is shipped at ambient temperature.
    Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    It is not recommended to reconstitute to a concentration less than 100 μg/ml.
    Dissolve the lyophilized protein in 1X PBS.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
    Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
    Aliquots of reconstituted samples are stable at < -20°C for 3 months.
    Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
    Endotoxin Less than 0.1 ng/µg (1 IEU/µg).
    Amino Acid Sequence
    IAENEDALLRHLFQGYQKWVRPVLHSNDTIKVYFGLKISQLVDVDEKNQLMTTNVWLKQEWTDHK LRWNPDDYGGIHSIKVPSESLWLPDIVLFENADGRFEGSLMTKVIVKSNGTVVWTPPASYKSSCT MDVTFFPFDRQNCSMKFGSWTYDGTMVDLILINENVDRKDFFDNGEWEILNAKGMKGNRRDGVYS YPFITYSFVLRRLLDHHHHHH*
    Background Neuronal acetylcholine receptor subunit beta-3(CHRNB3) is a cell membrane protein and belongs to the ligand-gated ion channel (TC 1.A.9) family. CHRNB3 seems to be composed of two different type of subunits: alpha and beta. The CHRNB3 are (hetero) pentamers composed of homologous subunits. The subunits that make up the muscle and neuronal forms of CHRNB3 are encoded by separate genes and have different primary structure. There are several subtypes of neuronal CHRNB3 that vary based on which homologous subunits are arranged around the central channel. They are classified as alpha-subunits if like muscle alpha-1, they have a pair of adjacent cysteines as part of the presumed acetylcholine binding site. Subunits lacking these cysteine residues are classified as beta-subunits.