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CC49
Recombinant Human Dkk-2/Dickkopf-related protein 2
10ug
840
756
现货
国产
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CC49
Recombinant Human Dkk-2/Dickkopf-related protein 2
50ug
2520
2268
现货
国产
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CC49
Recombinant Human Dkk-2/Dickkopf-related protein 2
500ug
12320
11088
现货
国产
-
CC49
Recombinant Human Dkk-2/Dickkopf-related protein 2
1mg
17600
15840
现货
国产
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Catalog# CC49 Source HEK293 Description Recombinant Human Dickkopf-related protein 2/Dkk-2 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Asp251-Ile526) of Human DKK2 fused with a polyhistidine tag at the C-terminus and a FC tag at the N-terminus. Names Dickkopf-related protein 2, Dickkopf-2, Dkk-2, hDkk-2 Accession # Q9UBU2 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.2 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence EPKSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVD GVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPRE PQVYTLPPSREEMTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKL TVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGKVDGGGSGGGSGGGSRKRRMAALMRSKDS SCCLLLLAAVLMVESSQIGSSRAKLNSIKSSLGGETPGQAANRSAGMYQGLAFGGSKKGKNLGQA YPCSSDKECEVGRYCHSPHQGSSACMVCRRKKKRCHRDGMCCPSTRCNNGICIPVTESILTPHIP ALDGTQHRDRNHGHYSNHDLGWQNLGRPHTKMSHIKGHEGDPCLRSSDCIEGFCCARHFWTKICK PVLHQGEVCTKQRKKGSHGLEIFQRCDCAKGLSCKVWKDATYSSKARLHVCQKIVDHHHHHHBackground Dickkopf-related protein 2 (DKK2), is a member of the dickkopf family. DKK2 is secreted protein which contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. It can act as either an agonist or antagonist of Wnt/beta-catenin signaling, depending on the cellular context and the presence of the co-factor kremen 2. It antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.