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CH36
Recombinant Human eIF-4E/Eukaryotic translation initiation factor 4E
10ug
640
576
现货
国产
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CH36
Recombinant Human eIF-4E/Eukaryotic translation initiation factor 4E
50ug
2000
1800
现货
国产
-
CH36
Recombinant Human eIF-4E/Eukaryotic translation initiation factor 4E
500ug
12320
11088
现货
国产
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CH36
Recombinant Human eIF-4E/Eukaryotic translation initiation factor 4E
1mg
17600
15840
现货
国产
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Catalog# CH36 Source E.coli Description Recombinant Human eIF-4E is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val217) of Human eIF-4E . Names Eukaryotic translation initiation factor 4E，eIF-4E，eIF-4F 25 kDa subunit，mRNA cap-binding protein，EIF4E，EIF4EL1，EIF4F Accession # P06730 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISK FDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFW LETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVI GYQSHADTATKSGSTTKNRFVVBackground Eukaryotic translation initiation factor 4E is a 217 amino acids protein that belongs to the eukaryotic initiation factor 4E family. eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners.It can recognize and bind the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.