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C600

Recombinant Human Endothelin-converting enzyme 1/ECE-1

10ug

1200

1080

现货

国产

C600

Recombinant Human Endothelin-converting enzyme 1/ECE-1

50ug

3520

3168

现货

国产

C600

Recombinant Human Endothelin-converting enzyme 1/ECE-1

500ug

12320

11088

现货

国产

C600

Recombinant Human Endothelin-converting enzyme 1/ECE-1

1mg

17600

15840

现货

国产

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  • Catalog# C600
    Source HEK293
    Description Recombinant Human Endothelin-Converting Enzyme 1/ECE-1 is produced with our mammalian expression system. The target protein is expressed with sequence (Gln90-Trp770) of Human ECE-1 fused with a polyhistidine tag at the C-terminus.
    Names Endothelin-Converting Enzyme 1, ECE-1, ECE1
    Accession # P42892
    Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 0.1mM ZnCl, pH 7.5
    Shipping The product is shipped on dry ice/ice packs.
    Storage Store at < -20°C, stable for 6 months after receipt.
    Please minimize freeze-thaw cycles.
    Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
    Endotoxin Less than 0.1 ng/μg (1 IEU/μg).
    Amino Acid Sequence
    QYQTRSPSVCLSEACVSVTSSILSSMDPTVDPCHDFFSYACGGWIKANPVPDGHSRWGTFSNLWE HNQAIIKHLLENSTASVSEAERKAQVYYRACMNETRIEELRAKPLMELIERLGGWNITGPWAKDN FQDTLQVVTAHYRTSPFFSVYVSADSKNSNSNVIQVDQSGLGLPSRDYYLNKTENEKVLTGYLNY MVQLGKLLGGGDEEAIRPQMQQILDFETALANITIPQEKRRDEELIYHKVTAAELQTLAPAINWL PFLNTIFYPVEINESEPIVVYDKEYLEQISTLINTTDRCLLNNYMIWNLVRKTSSFLDQRFQDAD EKFMEVMYGTKKTCLPRWKFCVSDTENNLGFALGPMFVKATFAEDSKSIATEIILEIKKAFEESL STLKWMDEETRKSAKEKADAIYNMIGYPNFIMDPKELDKVFNDYTAVPDLYFENAMRFFNFSWRV TADQLRKAPNRDQWSMTPPMVNAYYSPTKNEIVFPAGILQAPFYTRSSPKALNFGGIGVVVGHEL THAFDDQGREYDKDGNLRPWWKNSSVEAFKRQTECMVEQYSNYSVNGEPVNGRHTLGENIADNGG LKAAYRAYQNWVKKNGAEHSLPTLGLTNNQLFFLGFAQVWCSVRTPESSHEGLITDPHSPSRFRV IGSLSNSKEFSEHFRCPPGSPMNPPHKCEVWVDHHHHHH
    Background Endothelin-Converting Enzyme-1 (ECE-1) is a single-pass type I I transmembrane (TM) protein with a short cytoplasmic tail and a large ectodomain. ECE-1 is a zinc protease of the neprilysin (NEP) family, which also includes ECE-2, PEX, XCE, DINE, and Kell, and several NEP-like proteins. It is widely expressed and has several alternatively spliced forms that differ in their TM domain or cytoplasmic tail. All isoforms of ECE-1 are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Endothelin-converting enzyme-1 is involved in the proteolytic processing of Endothelin-1 (EDN1), Endothelin-2 (EDN2), and Endothelin-3 (EDN3) to biologically active peptides. Defects in ECE1 are a cause of Hirschsprung disease, cardiac defects and autonomic dysfunction (HSCRCDAD). It is a form of Hirschsprung disease with skip-lesions defects, craniofacial abnormalities and other dysmorphic features, and autonomic dysfunction.