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C318
Recombinant Human CD32a/Fc gamma RII
10ug
640
576
现货
国产
-
C318
Recombinant Human CD32a/Fc gamma RII
50ug
1760
1584
现货
国产
-
C318
Recombinant Human CD32a/Fc gamma RII
500ug
12320
11088
现货
国产
-
C318
Recombinant Human CD32a/Fc gamma RII
1mg
17600
15840
现货
国产
-
C318
Recombinant Human Fc-gamma RII-a/CD32a
10ug
640
576
现货
国产
-
C318
Recombinant Human Fc-gamma RII-a/CD32a
50ug
1760
1584
现货
国产
-
C318
Recombinant Human Fc-gamma RII-a/CD32a
500ug
12320
11088
现货
国产
-
C318
Recombinant Human Fc-gamma RII-a/CD32a
1mg
17600
15840
现货
国产
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Catalog# C318 Source HEK293 Description Recombinant Human FcγRIIA/FCGR2A is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Gln34-Val219) of Human FCGR2A fused with a polyhistidine tag at the C-terminus. Names Low Affinity Immunoglobulin Gamma Fc Region Receptor II-a, IgG Fc receptor II-a, CDw32, Fc-Gamma RII-a, Fc-Gamma-RIIa, FcRII-a, CD32, FCGR2A, CD32, FCG2, FCGR2A1, IGFR2 Accession # P12318 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence QAAPPKAVLKLEPPWINVLQEDSVTLTCQGARSPESDSIQWFHNGNLIPTHTQPSYRFKANNNDS GEYTCQTGQTSLSDPVHLTVLSEWLVLQTPHLEFQEGETIMLRCHSWKDKPLVKVTFFQNGKSQK FSRLDPTFSIPQANHSHSGDYHCTGNIGYTLFSSKPVTITVQVPSMGSSSPMGIIVDHHHHHHBackground Receptors for the Fc region of IgG (FcγR) are members of the Ig superfamily that function in the activation or inhibition of immune responses. Human FcγRs are divided into three classes designated FcγRI (CD64), FcγRII (CD32), and FcγRIII (CD16), which generate multiple isoforms, are recognized. The activating- type receptor either has or associates non-covalently with an accessory subunit that has an immunoreceptor tyrosine-based activation motif (ITAM) in its cytoplasmic domain. FcγRI binds IgG with high affinity and functions during early immune responses, whereas FcγRII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses. Three genes for human FcγRII (A, B, and C) and one for mouse (FcγRIIB), encoding type I transmembrane proteins with ITAM motifs (FcγRII A and C) or ITIM motifs (FcγRIIB) in their cytoplasmic domains, have been identified. Human CD32, also known as Low affinity immunoglobulin γ Fc region receptor II-a (IgG Fc receptor II-a), FcγRII A or FCGR2A Protein, is expressed on cells of both myeloid and lymphoid lineages as well as on cells of non-hematopoietic origin. Associated with an ITAM-bearing adapter subunit, FcRγ, CD32a (FcγRII A) delivers an activating signal upon ligand binding, and results in the initiation of inflammatory responses including cytolysis, phagocytosis, degranulation, and cytokine production. The responses can be modulated by signals from the co-expressed inhibitory receptors such as Fcγ RII B, and the strength of the signal is dependent on the ratio of expression of the activating and inhibitory receptors.
References Kustiawan I,et al.Preventing adsorption of immunoglobulin G to solid surfaces using poloxamer 407 eliminates artifactual stimulation of neutrophils
PMID:23545494
http://www.ncbi.nlm.nih.gov/pubmed/23545494