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C140
Recombinant Human Flap endonuclease 1/FEN-1
10ug
840
756
现货
国产
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C140
Recombinant Human Flap endonuclease 1/FEN-1
50ug
2520
2268
现货
国产
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C140
Recombinant Human Flap endonuclease 1/FEN-1
500ug
12320
11088
现货
国产
-
C140
Recombinant Human Flap endonuclease 1/FEN-1
1mg
17600
15840
现货
国产
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Catalog# C140 Source E.coli Description Recombinant Human Flap Endonuclease 1/FEN-1 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys380) of Human FEN-1. Names Flap Endonuclease 1, FEN-1, DNase IV, Flap Structure-Specific Endonuclease 1, Maturation Factor 1, MF1, hFEN-1, FEN1, RAD2 Accession # P39748 Formulation Supplied as a 0.2 μm filtered solution of 50 mM Tris, 50mM NaCl, 1mM DDT, 10% Glycerol, pH 8.0 Shipping The product is shipped on dry ice/ice packs. Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence MGIQGLAKLIADVAPSAIRENDIKSYFGRKVAIDASMSIYQFLIAVRQGGDVLQNEEGETTSHLM GMFYRTIRMMENGIKPVYVFDGKPPQLKSGELAKRSERRAEAEKQLQQAQAAGAEQEVEKFTKRL VKVTKQHNDECKHLLSLMGIPYLDAPSEAEASCAALVKAGKVYAAATEDMDCLTFGSPVLMRHLT ASEAKKLPIQEFHLSRILQELGLNQEQFVDLCILLGSDYCESIRGIGPKRAVDLIQKHKSIEEIV RRLDPNKYPVPENWLHKEAHQLFLEPEVLDPESVELKWSEPNEEELIKFMCGEKQFSEERIRSGV KRLSKSRQGSTQGRLDDFFKVTGSLSSAKRKEPEPKGSTKKKAKTGAAGKFKRGKBackground Flap Endonuclease 1 (FEN1) is a member of the XPG/RAD2 endonuclease family. During DNA replication, FEN1 cleaves the 5'-overhanging flap structure and processes the 5' ends of Okazaki fragments for synthesis. FEN1 also exhibits RNase H activity by possessing 5'-3' exonuclease activity on gapped double-stranded or nicked DNA, FEN1 is involved in the long patch base excision repair (LP-BER) pathway, it can cleave within the apurinic/apyrimidinic (AP) site-terminated flap. FEN1 can prevent flaps from equilibrating into structures that lead to duplications and deletions. FEN1 is also involved in replication and repair of rDNA and in repairing mitochondrial DNA.