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C142
Recombinant Human Fpg/Formamidopyrimidine-DNA glycosylase
10ug
840
756
现货
国产
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C142
Recombinant Human Fpg/Formamidopyrimidine-DNA glycosylase
50ug
2520
2268
现货
国产
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C142
Recombinant Human Fpg/Formamidopyrimidine-DNA glycosylase
500ug
12320
11088
现货
国产
-
C142
Recombinant Human Fpg/Formamidopyrimidine-DNA glycosylase
1mg
17600
15840
现货
国产
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Catalog# C142 Source E.coli Description Recombinant E. coli Formamidopyrimidine-DNA Glycosylase/Fpg is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys269) of E. coli Fpg. Names Formamidopyrimidine-DNA Glycosylase, Fapy-DNA Glycosylase, DNA-(Apurinic or Apyrimidinic Site) Lyase MutM, AP Lyase MutM, mutM, fpg Accession # A7ZTI6 Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, 1mM EDTA, 1mM DTT, 50% Glycerol, pH 7.8 Shipping The product is shipped on dry ice/ice packs. Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPE GWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLG PEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAEC ELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRA TFYCRQCQKLEHHHHHHBackground Fpg is a DNA glycosylase that releases damaged bases preferentially from duplex DNA. It has an associated class I AP (apurinic/apyrimidinic) lyase activity.Fpg Cleaves the DNA backbone to generate a single-strand break at the site of the removed base, The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving 3' and 5' phosphoryl groups.