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C657
Recombinant Human Gastric triacylglycerol lipase/LIPF
10ug
1200
1080
现货
国产
-
C657
Recombinant Human Gastric triacylglycerol lipase/LIPF
50ug
3520
3168
现货
国产
-
C657
Recombinant Human Gastric triacylglycerol lipase/LIPF
500ug
12320
11088
现货
国产
-
C657
Recombinant Human Gastric triacylglycerol lipase/LIPF
1mg
17600
15840
现货
国产
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Catalog# C657 Source HEK293 Description Recombinant Human Gastric Triacylglycerol Lipase/LIPF is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Leu20-Lys398) of Human LIPF fused with a polyhistidine tag at the C-terminus. Names Gastric Triacylglycerol Lipase, GL, Gastric Lipase, LIPF Accession # P07098 Formulation Lyophilized from a 0.2 μm filtered solution of 25mM TrisHCl, 100mM glycine, 10% glycerol,pH7.3 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence LFGKLHPGSPEVTMNISQMITYWGYPNEEYEVVTEDGYILEVNRIPYGKKNSGNTGQRPVVFLQH GLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYD LPATIDFIVKKAGQKQLHYVGHSQGTTIGFIAFSTNPSLAKRIKTFYALAPVATVKYTKSLINKL RFVPQSLFKFIFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYL SHNPAGTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDL LADPQDVGLLLPKLPNLIYHKEIPFYNHLDFIWAMDAPQEVYNDIVSMISEDKKVDHHHHHHBackground Gastric Triacylglycerol Lipase (LIPF) belongs to the AB hydrolase superfamily. LIPF is an important lipase during the digestion of dietary lipids in cystic fibrosis. LIPF is involved in the digestion of dietary triglycerides in the gastrointestinal tract, and responsible for 30% of fat digestion processes occurring in human. LIPF is secreted by gastric chief cells in the fundic mucosa of the stomach, and it hydrolyzes the ester bonds of triglycerides under acidic pH conditions. LIPF acts distinct roles in neutral lipid metabolism.