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C144
Recombinant Human Grancalcin/GCA(GST fusion)
10ug
840
756
现货
国产
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C144
Recombinant Human Grancalcin/GCA(GST fusion)
50ug
2520
2268
现货
国产
-
C144
Recombinant Human Grancalcin/GCA(GST fusion)
500ug
12320
11088
现货
国产
-
C144
Recombinant Human Grancalcin/GCA(GST fusion)
1mg
17600
15840
现货
国产
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Catalog# C144 Source E.coli Description Recombinant Human Grancalcin/GCA is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys218) of Human Grancalcin fused with a GST tag at the N-terminus. Names Grancalcin, GCA, GCL Accession # P28676 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM Nacl, pH 8.5 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKL TQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEML KMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKS SKYIAWPLQGWQATFGGGDHPPKSDLVPRGSMAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAIL LDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIA MLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIV KRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAIBackground Grancalcin (GCA) is a member of the penta EF hand subfamily which includes sorcin, calpain and ALG2. Grancalcin is highly expressed bone marrow and also can detected in neutrophils and macrophages. Grancalcin interacts with L-plastin which known to have actin bundling activity. It indicates that Grancalcin may play an important role in the adhesion of neutrophils to fibronectin. Furthermore, Grancalcin localization is dependent upon calcium and magnesium. It associates with both the granule and membrane fractions, which suggested a role for grancalcin in granule-membrane fusion and degranulation.