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CE35
Recombinant Human Growth factor receptor-bound protein 2/GRB2/ASH
10ug
1200
1080
现货
国产
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CE35
Recombinant Human Growth factor receptor-bound protein 2/GRB2/ASH
50ug
3520
3168
现货
国产
-
CE35
Recombinant Human Growth factor receptor-bound protein 2/GRB2/ASH
500ug
12320
11088
现货
国产
-
CE35
Recombinant Human Growth factor receptor-bound protein 2/GRB2/ASH
1mg
17600
15840
现货
国产
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Catalog# CE35 Source E.coli Description Recombinant Human Growth Factor Receptor-Bound Protein 2/GRB2 is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Val217) of Human GRB2 fused with a 6His tag at the C-terminus. Names Growth Factor Receptor-Bound Protein 2, Adapter Protein GRB2, Protein Ash, SH2/SH3 Adapter GRB2, GRB2, ASH Accession # P62993 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence MEAIAKYDFKATADDELSFKRGDILKVLNEECDQNWYKAELNGKDGFIPKNYIEMKPHPWFFGKI PRAKAEEMLSKQRHDGAFLIRESESAPGDFSLSVKFGNDVQHFKVLRDGAGKYFLWVVKFNSLNE LVDYHRSTSVSRNQQIFLRDIEQVPQQPTYVQALFDFDPQEDGELGFRRGDFIHVMDNSDPNWWK GACHGQTGMFPRNYVTPVNRNVLEHHHHHHBackground As an adaptor protein, Growth Factor Receptor-Bound Protein 2 (GRB2) is involved in siganl transduction and consists of a central SH2 domain flanked by two SH3 domains. GRB2 associates with activated Tyr-phosphorylated EGF receptor/EGFR and PDGF receptors via its SH2 domain, stimulating GTP binding to Ras, which in turn activates MAPK and other signaling pathway.It also associates to other cellular Tyr-phosphorylated proteins such as SIT1, IRS1, IRS4, SHC and LNK. probably via the concerted action of both its SH2 and SH3 domains.