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C971

Recombinant Human Histidine ammonia-lyase/HAL

10ug

1200

1080

现货

国产

C971

Recombinant Human Histidine ammonia-lyase/HAL

50ug

3520

3168

现货

国产

C971

Recombinant Human Histidine ammonia-lyase/HAL

500ug

12320

11088

现货

国产

C971

Recombinant Human Histidine ammonia-lyase/HAL

1mg

17600

15840

现货

国产

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  • Catalog# C971
    Source HEK293
    Description Recombinant Human Histidase/HAL is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Met1-Leu657) of Human HAL fused with a 6His tag at the C-terminus.
    Names Histidine Ammonia-Lyase, Histidase, HAL, HIS
    Accession # P42357
    Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 10% Glycerol, pH 7.5
    Shipping The product is shipped on dry ice/ice packs.
    Storage Store at < -20°C, stable for 6 months after receipt.
    Please minimize freeze-thaw cycles.
    Purity Greater than 95% as determined by reducing SDS-PAGE.
    Endotoxin Less than 0.1 ng/μg (1 IEU/μg).
    Amino Acid Sequence
    MPRYTVHVRGEWLAVPCQDAQLTVGWLGREAVRRYIKNKPDNGGFTSVDDAHFLVRRCKGLGLLD NEDRLEVALENNEFVEVVIEGDAMSPDFIPSQPEGVYLYSKYREPEKYIELDGDRLTTEDLVNLG KGRYKIKLTPTAEKRVQKSREVIDSIIKEKTVVYGITTGFGKFARTVIPINKLQELQVNLVRSHS SGVGKPLSPERCRMLLALRINVLAKGYSGISLETLKQVIEMFNASCLPYVPEKGTVGASGDLAPL SHLALGLVGEGKMWSPKSGWADAKYVLEAHGLKPVILKPKEGLALINGTQMITSLGCEAVERASA IARQADIVAALTLEVLKGTTKAFDTDIHALRPHRGQIEVAFRFRSLLDSDHHPSEIAESHRFCDR VQDAYTLRCCPQVHGVVNDTIAFVKNIITTELNSATDNPMVFANRGETISGGNFHGEYPAKALDY LAIGIHELAAISERRIERLCNPSLSELPAFLVAEGGLNSGFMIAHCTAAALVSENKALCHPSSVD SLSTSAATEDHVSMGGWAARKALRVIEHVEQVLAIELLAACQGIEFLRPLKTTTPLEKVYDLVRS VVRPWIKDRFMAPDIEAAHRLLLEQKVWEVAAPYIEKYRMEHIPESRPLSPTAFSLQFLHKKSTK IPESEDLVDHHHHHH
    Background Histidase (HAL) belongs to the PAL/Histidase family. Histidase deaminates histidine to urocanic acid, the first step in histidine degradation. It is closely related to the plant enzyme phenylalanine ammonia-lyase , but is absent in plants and viruses. HAL contains a unique cofactor called 4-methylidene-imidazole-5-one group (MIO), which is produced autocatalytically by a cyclization and dehydration of the three amino-acid residues alanine, serine and glycine, for its chain folding. Defects in HAL are the cause of histidinemia (HISTID). HISTID is characterized by decreased urocanic acid as well as increased histamine and histidine in body fluids.