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CE66
Recombinant Human HMGB1/High mobility group protein B1/HMG-1
10ug
1024
921.6
现货
国产
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CE66
Recombinant Human HMGB1/High mobility group protein B1/HMG-1
50ug
3080
2772
现货
国产
-
CE66
Recombinant Human HMGB1/High mobility group protein B1/HMG-1
500ug
12320
11088
现货
国产
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CE66
Recombinant Human HMGB1/High mobility group protein B1/HMG-1
1mg
17600
15840
现货
国产
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Catalog# CE66 Source E.coli Description Recombinant Human High Mobility Group Protein B1/HMGB1/BOXA is produced by our E. coli expression system. The target protein is expressed with sequence (G2-F89) of Human HMBG1. Names High-mobility group protein B1, high-mobility group protein 1, HMG-1, amphoterin and HMGB1. Accession # P09429 Formulation Lyophilized from a 0.2 μm filtered solution of 50mM HEPES,500mM NaCl,0.5mM DTT, pH7.9 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence GKGDPKKPRGKMSSYAFFVQTCREEHKKKHPDASVNFSEFSKKCSERWKTMSAKEKGKFEDMAKA DKARYEREMKTYIPPKGETKKKF*Background HMGB1 belongs to the HMGB family and contains 2 HMG box DNA-binding domains. HMGB1 is a DNA binding proteins that associates with chromatin and has the ability to bend DNA. This nuclear protein organizes the DNA and regulates transcription. After binding HMGB1 bends the DNA, which conducts to the binding of other proteins. HMGB1 suports transcription of many genes in interactions with many transcription factors or cooperation nucleosoms looses packed DNA and increases the chromatin remodeling, contact with core histones changes the structure of nucleosoms. it involved in V(D)J recombination by acting as a cofactor of the RAG complex. The presence of HMGB1 in the nucleus depends on posttranslational modifications. When the protein is not acetylated, stays in the nucleus, but hyperacetylation on lysine residues occurs to translocation into the cytosol.