||Recombinant Human Interleukin-2/IL-2 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Pro22-Thr153) of Human IL-2 fused with a polyhistidine tag at the C-terminus.
||Interleukin-2, IL-2, T-Cell Growth Factor, TCGF, Aldesleukin, IL2
||Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH 7.4
||The product is shipped at ambient temperature.
||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
||Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
||Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
||Less than 0.1 ng/µg (1 IEU/µg).
|Amino Acid Sequence
PTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPL EEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIIST LTVDHHHHHH
||Recombinant Human Interleukin-2 is a highly purified protein with a molecular weight of approximately 15,300 Daltons. The chemical name is des-alanyl-1, serine-125 Human Interleukin-2. It is produced by recombinant DNA technology using a genetically engineered E. coli strain containing an analog of the human interleukin-2 gene. Genetic engineering techniques were used to modify the Human IL-2 gene, and the resulting expression clone encodes a modified Human IL-2. This recombinant form differs from native Interleukin-2 in following ways: 1) it is not glycosylated; 2) the molecule has no N-terminal alanine; 3) the molecule has serine substituted for cysteine at amino acid position 125; 4) the aggregation state of molecule is likely to be different from that of native IL-2.