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C017
Recombinant Human LIF/Leukemia inhibitory factor
10ug
840
756
现货
国产
-
C017
Recombinant Human LIF/Leukemia inhibitory factor
50ug
2520
2268
现货
国产
-
C017
Recombinant Human LIF/Leukemia inhibitory factor
500ug
12800
11520
现货
国产
-
C017
Recombinant Human LIF/Leukemia inhibitory factor
1mg
21120
19008
现货
国产
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Catalog# C017 Source E.coli Description Recombinant Human LIF, Leukemia Inhibitory Factor produced in E. coli is a single non-glycosylated polypeptide chain containing 180 amino acids with a molecular mass of 19.7 kD. Names Recombinant Human LIF, Leukemia Inhibitory Factor, LIF, Differentiation-Stimulating Factor, D Factor, Melanoma-Derived LPL Inhibitor, MLPLI, Emfilermin, LIF, HILDA Accession # P15018 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, 0.02% Tween 20, pH 7.4 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Biological Activity ED50 is less than 0.01 ng/ml as determined by the M1 cell differentiation assay.
Specific Activity of 1.0 x 108 IU/mg.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence MSPLPITPVNATCAIRHPCHNNLMNQIRSQLAQLNGSANALFILYYTAQGEPFPNNLDKLCGPNV TDFPPFHANGTEKAKLVELYRIVVYLGTSLGNITRDQKILNPSALSLHSKLNATADILRGLLSNV LCRLCSKYHVGHVDVTYGPDTSGKDVFQKKKLGCQLLGKYKQIIAVLAQAFBackground Leukemia Inhibitory Factor (LIF) is a lymphoid factor that promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation. LIF has a number of other activities including cholinergic neuron differentiation, control of stem cell pluripotency, bone and fat metabolism, mitogenesis of certain factor dependent cell lines and promotion of megakaryocyte production in vivo. Human and murine mature LIF exhibit a 78% sequence identity at the amino acid level. Human LIF is equally active on human and mouse cells. Murine LIF is approximately 1000 fold less active on human cells than human LIF.