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C917
Recombinant Human MECR/Trans-2-enoyl-CoA reductase
10ug
1200
1080
现货
国产
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C917
Recombinant Human MECR/Trans-2-enoyl-CoA reductase
50ug
3520
3168
现货
国产
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C917
Recombinant Human MECR/Trans-2-enoyl-CoA reductase
500ug
12320
11088
现货
国产
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C917
Recombinant Human MECR/Trans-2-enoyl-CoA reductase
1mg
17600
15840
现货
国产
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Catalog# C917 Source Human Cells Description Recombinant Human Trans-2-Enoyl-CoA Reductase Mitochondrial/MECR is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (Pro54-Met373) of Human MECR fused with a polyhistidine tag at the C-terminus. Names Trans-2-Enoyl-CoA Reductase Mitochondrial, Nuclear Receptor-Binding Factor 1, HsNrbf-1NRBF-1, MECR, NBRF1 Accession # Q9BV79 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence PAKVVELKNLELAAVRGSDVRVKMLAAPINPSDINMIQGNYGLLPELPAVGGNEGVAQVVAVGSN VTGLKPGDWVIPANAGLGTWRTEAVFSEEALIQVPSDIPLQSAATLGVNPCTAYRMLMDFEQLQP GDSVIQNASNSGVGQAVIQIAAALGLRTINVVRDRPDIQKLSDRLKSLGAEHVITEEELRRPEMK NFFKDMPQPRLALNCVGGKSSTELLRQLARGGTMVTYGGMAKQPVVASVSLLIFKDLKLRGFWLS QWKKDHSPDQFKELILTLCDLIRRGQLTAPACSQVPLQDYQSALEASMKPFISSKQILTMVDHHH HHHBackground Trans-2-Enoyl-CoA Reductase Mitochondrial (MECR) belongs to the zinc-containing alcohol dehydrogenase family. MECR localizes to the mitochondrion. It is highly expressed in skeletal and heart muscle and expressed at lower levels in the placenta, liver, kidney and pancreas, with weakly or no expression in the lung. MECR exists as a homodimer, which catalyzes the reduction of trans-2-enoyl-CoA to acyl-CoA with chain length from C6 to C16 in an NADPH-dependent manner with preference to medium chain length substrate. MECR may take part in the mitochondrial synthesis of fatty acids.