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CG86

Recombinant Human Methylated-DNA--protein-cysteine methyltransferase /MGMT

10ug

760

684

现货

国产

CG86

Recombinant Human Methylated-DNA--protein-cysteine methyltransferase /MGMT

50ug

2280

2052

现货

国产

CG86

Recombinant Human Methylated-DNA--protein-cysteine methyltransferase /MGMT

500ug

12320

11088

现货

国产

CG86

Recombinant Human Methylated-DNA--protein-cysteine methyltransferase/MGMT

1mg

17600

15840

现货

国产

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  • Catalog# CG86
    Source E.coli
    Description Recombinant Human Methylated-DNA--protein-cysteine methyltransferase/MGMT is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Asn207) of Human MGMT fused with a 6His tag at the N-terminus.
    Names ' Methylated-DNA--protein-cysteine methyltransferase, 6-O-methylguanine-DNAmethyltransferase,O-6-methylguanine-DNA-alkyltransferase, MGMT'
    Accession # P16455
    Formulation Supplied as a 0.2 μm filtered solution of 20mMTris-HCl,1mM EDTA, pH 8.0
    Shipping The product is shipped on dry ice/ice packs.
    Storage Store at < -20°C, stable for 6 months after receipt.
    Please minimize freeze-thaw cycles.
    Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.
    Endotoxin Less than 0.1 ng/µg (1 IEU/µg).
    Amino Acid Sequence
    MGSSHHHHHHSSGLVPRGSHMDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE VPAPAAVLGGPEPLMQCTAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLKVVKFGE VISYQQLAALAGNPKAARAVGGAMRGNPVPILIPCHRVVCSSGAVGNYSGGLAVKEWLLAHEGHR LGKPGLGGSSGLAGAWLKGAGATSGSPPAGRN
    Background 'MGMT belongs to the family of transferases, specifically those transferring one-carbon group methyltransferases. MGMT involved in the cellular defense against the biological effects of O6-methylguanine in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. MGMT catalyzes the chemical reaction: DNA (containing 6-O-methylguanine) and proteinL-cysteine into DNA (without 6-O-methylguanine) and protein S-methyl-L-cysteine.'