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C492
Recombinant Human Nectin-1/PVRL1
10ug
640
576
现货
国产
-
C492
Recombinant Human Nectin-1/PVRL1
50ug
1760
1584
现货
国产
-
C492
Recombinant Human Nectin-1/PVRL1
500ug
12320
11088
现货
国产
-
C492
Recombinant Human Nectin-1/PVRL1
1mg
17600
15840
现货
国产
-
C492
Recombinant Human PVRL1/Nectin-1
10ug
640
576
现货
国产
-
C492
Recombinant Human PVRL1/Nectin-1
50ug
1760
1584
现货
国产
-
C492
Recombinant Human PVRL1/Nectin-1
500ug
12320
11088
现货
国产
-
C492
Recombinant Human PVRL1/Nectin-1
1mg
17600
15840
现货
国产
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Catalog# C492 Source HEK293 Description Recombinant Human Poliovirus Receptor-Related Protein 1/PVRL1 is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Gln31-Thr334) of Human PVRL1 fused with a polyhistidine tag at the C-terminus. Names Poliovirus Receptor-Related Protein 1, Herpes Virus Entry Mediator C, Herpesvirus Entry Mediator C, HveC, Herpesvirus Ig-Like Receptor, HIgR, Nectin-1, CD111, PVRL1, HVEC, PRR1 Accession # Q15223 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence QVVQVNDSMYGFIGTDVVLHCSFANPLPSVKITQVTWQKSTNGSKQNVAIYNPSMGVSVLAPYRE RVEFLRPSFTDGTIRLSRLELEDEGVYICEFATFPTGNRESQLNLTVMAKPTNWIEGTQAVLRAK KGQDDKVLVATCTSANGKPPSVVSWETRLKGEAEYQEIRNPNGTVTVISRYRLVPSREAHQQSLA CIVNYHMDRFKESLTLNVQYEPEVTIEGFDGNWYLQRMDVKLTCKADANPPATEYHWTTLNGSLP KGVEAQNRTLFFKGPINYSLAGTYICEATNPIGTRSGQVEVNITVDHHHHHHBackground Nectin-1 is a type I transmembrane glycoprotein belonging to the Ig superfamily. Nectin-1 promotes cell-cell contacts by forming homophilic or heterophilic trans-dimers. Heterophilic interactions have been detected between Nectin-1 and Nectin-3 and between Nectin-1 and Nectin-4. Nectin ECDs contain three Ig like domains: an N terminal V type that mediates ligand binding, and two C2 type. Nectin-1 binds viral Glycoprotein D to mediate Herpesvirus (but not Poxvirus) entry into vaginal mucosa, sensory neurons and fibroblasts. In forming adherens junctions and synapses, Nectin-1 and Nectin-3 initiate cell-cell interactions, recruiting αvβ3 integrin extracellularly and cadherins intracellularly through afadin and other junctional proteins. These interactions organize the cytoskeleton, strengthen attachment to basement membrane and promote further cell-cell connections. Nectin-1 and Nectin-3 have been found to localize assymetrically along the chemical synapse, with Nectin-1 primarily on the axonal side and Nectin-3 on the dendritic side. Deficiency of Nectin-1 can result in cleft lip/palate ectodermal dysplasia. Nectin-1 downregulation in epithelial cancers is mediated in part by ectodomain shedding, but it may contribute to invasiveness.