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C388
Recombinant Human ON/SPARC/BM-40
10ug
400
360
现货
国产
-
C388
Recombinant Human ON/SPARC/BM-40
50ug
1120
1008
现货
国产
-
C388
Recombinant Human ON/SPARC/BM-40
500ug
4920
4428
现货
国产
-
C388
Recombinant Human ON/SPARC/BM-40
1mg
7040
6336
现货
国产
-
C388
Recombinant Human SPARC/ON/BM-40
10ug
400
360
现货
国产
-
C388
Recombinant Human SPARC/ON/BM-40
50ug
1120
1008
现货
国产
-
C388
Recombinant Human SPARC/ON/BM-40
500ug
4920
4428
现货
国产
-
C388
Recombinant Human SPARC/ON/BM-40
1mg
7040
6336
现货
国产
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Catalog# C388 Source HEK293 Description Recombinant Human SPARC is produced with our mammalian expression system in human cells. The target protein is expressed with sequence (Ala18-Ile303) of Human SPARC fused with a polyhistidine tag at the C-terminus. Names SPARC, Basement-Membrane Protein 40, BM-40, Osteonectin, ON, Secreted Protein Acidic and Rich in Cysteine, SPARC, ON Accession # P09486 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence APQQEALPDETEVVEETVAEVTEVSVGANPVQVEVGEFDDGAEETEEEVVAENPCQNHHCKHGKV CELDENNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIG PCKYIPPCLDSELTEFPLRMRDWLKNVLVTLYERDEDNNLLTEKQKLRVKKIHENEKRLEAGDHP VELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDN DKYIALDEWAGCFGIKQKDIDKDLVIVDHHHHHHBackground Secreted Protein Acidic and Rich in Cysteine (SPARC) is a secreted, evolutionarily conserved collagen-binding glycoprotein and belongs to the SPARC family. SPARC has 286 amino acids and contains an EF-hand in C-termina domain, a follistatin-like domain with Kazal-like sequences. There are two calcium binding sites, one binds 5 - 8 Ca2+ with a low affinity and other on an EF-hand loop that binds a Ca2+ ion with a high affinity. It is highly expressed in tissues undergoing morphogenesis, remodeling and wound repair. SPARC regulate cell growth through interactions with the extracellular matrix (ECM) and cytokines. SPARC bind to numerous proteins of the ECM, affect ECM protein expression, influence cellular adhesion and migration, and modulate growth factor-induced cell proliferation and angiogenesis. SPARC also binds several types of collagen, albumin, thrombospondin, PDGF and cell membranes.