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C173
Recombinant Human Stratifin/SFN/14-3-3 protein σ
10ug
840
756
现货
国产
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C173
Recombinant Human Stratifin/SFN/14-3-3 protein σ
50ug
2520
2268
现货
国产
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C173
Recombinant Human Stratifin/SFN/14-3-3 protein σ
500ug
12320
11088
现货
国产
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C173
Recombinant Human Stratifin/SFN/14-3-3 protein σ
1mg
17600
15840
现货
国产
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Catalog# C173 Source E.coli Description Recombinant Human Stratifin is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Ser248) of Human Stratifin. Names 14-3-3 Protein Sigma, Epithelial Cell Marker Protein 1, Stratifin, SFN, HME1 Accession # P31947 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, 1mM EDTA, 1mM DTT, pH 7.4 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSI EQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRY LAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAK TTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQSBackground Stratifin (SFN) belongs to the 14-3-3 family of proteins that act as important regulators of intracelluar signal transduction through their ability to bind specific motifs phosphorylated on serine or threonine. There are at least seven isoforms that have been identified in mammals (beta, gamma, epsilon, sigma, zeta, tau and eta). SFN can detected in many tissues, highly expressed in stratified squamous keratinizing epithelium. SFN is indicated as an epithelial cell marker and serves as a tumor suppressor whose expression can be down regulated by methylation. In addition, SFN plays a key role in maintaining the G2 checkpoint in cells and preventing mitotic death.