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C293
Recombinant Human Transcription initiation factor IIB/GTF2B
10ug
1200
1080
现货
国产
-
C293
Recombinant Human Transcription initiation factor IIB/GTF2B
50ug
3520
3168
现货
国产
-
C293
Recombinant Human Transcription initiation factor IIB/GTF2B
500ug
12320
11088
现货
国产
-
C293
Recombinant Human Transcription initiation factor IIB/GTF2B
1mg
17600
15840
现货
国产
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Catalog# C293 Source E.coli Description Recombinant Human Transcription Initiation Factor IIB/TFIIB is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Leu316) of Human TFIIB fused with a GST tag at the N-terminus. Names Transcription Initiation Factor IIB, General Transcription Factor TFIIB, S300-II, GTF2B, TF2B, TFIIB Accession # Q00403 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 100mM NaCl, pH 8.0 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKL TQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEML KMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKS SKYIAWPLQGWQATFGGGDHPPKSDLVPRGSPEFHMASTSRLDALPRVTCPNHPDAILVEDYRAG DMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAASFDE FGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIA SACLYIACRQEGVPRTFKEICAVSRISKKEIGRCFKLILKALETSVDLITTGDFMSRFCSNLCLP KQVQMAATHIARKAVELDLVPGRSPISVAAAAIYMASQASAEKRTQKEIGDIAGVADVTIRQSYR LIYPRAPDLFPTDFKFDTPVDKLPQLBackground Transcription Initiation Factor IIB (TFIIB) is an essential factor for transcription by RNA Polymerase II. TFIIB localizes to the nucleus where it forms a complex (the DAB complex) with transcription factor IID and IIA. TFIIB plays a role as a bridge between IID, which initially recognizes the promoter sequence, and RNA polymerase II. TFIIB is involved in the selection of transcription start site.