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CG49
Recombinant Human UBE2M/NEDD8-conjugating enzyme Ubc12/UBC12
10ug
240
216
现货
国产
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CG49
Recombinant Human UBE2M/NEDD8-conjugating enzyme Ubc12/UBC12
50ug
720
648
现货
国产
-
CG49
Recombinant Human UBE2M/NEDD8-conjugating enzyme Ubc12/UBC12
500ug
4800
4320
现货
国产
-
CG49
Recombinant Human UBE2M/NEDD8-conjugating enzyme Ubc12/UBC12
1mg
7600
6840
现货
国产
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Catalog# CG49 Source E.coli Description Recombinant Human NEDD8-conjugating enzyme Ubc12/UBE2M is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Lys183) of Human UBE2M. Names NEDD8-conjugating enzyme Ubc12, NEDD8 carrier protein, NEDD8 protein ligase, Ubiquitin-conjugating enzyme E2 M, UBC12, UBE2M, Accession # P61081 Formulation Supplied as a 0.2 μm filtered solution of 50mM HEPES,2mM DTT,150mM NaCl,10%glycerol,pH 7.5 Shipping The product is shipped on dry ice/ice packs. Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence MIKLFSLKQQKKEEESAGGTKGSSKKASAAQLRIQKDINELNLPKTCDISFSDPDDLLNFKLVIC PDEGFYKSGKFVFSFKVGQGYPHDPPKVKCETMVYHPNIDLEGNVCLNILREDWKPVLTINSIIY GLQYLFLEPNPEDPLNKEAAEVLQNNRRLFEQNVQRSMRGGYIGSTYFERCLKBackground UBE2M is a member of the E2 ubiquitin-conjugating enzyme family. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. UBE2M accepts the ubiquitin-like protein NEDD8 from the UBA3-NAE1 E1 complex and catalyzes its covalent attachment to other proteins. The specific interaction with the E3 ubiquitin ligase RBX1, but not RBX2, suggests that the RBX1-UBE2M complex neddylates specific target proteins, such as CUL1, CUL2, CUL3 and CUL4. It involved in cell proliferation and is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.