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CH07
Recombinant Human Ubiquitin-like modifier-activating enzyme 5/UBA5
10ug
840
756
现货
国产
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CH07
Recombinant Human Ubiquitin-like modifier-activating enzyme 5/UBA5
50ug
2520
2268
现货
国产
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CH07
Recombinant Human Ubiquitin-like modifier-activating enzyme 5/UBA5
500ug
12320
11088
现货
国产
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CH07
Recombinant Human Ubiquitin-like modifier-activating enzyme 5/UBA5
1mg
17600
15840
现货
国产
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Catalog# CH07 Source E.coli Description Recombinant Human Ubiquitin-like modifier-activating enzyme 5/UBA5 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Met404) of Human UBA5 fused with a 6His tag at the N-terminus. Names Ubiquitin-like modifier-activating enzyme 5, ThiFP1, UFM1-activating enzyme, Ubiquitin-activating enzyme E1 domain-containing protein 1, UBE1DC1, UBA5 Accession # Q9GZZ9 Formulation Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl pH 8.0,1mM DTT, 10% glycerol, 50mM NaCl Shipping The product is shipped on dry ice/ice packs. Storage Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence MGSSHHHHHHSSGLVPRGSHMAESVERLQQRVQELERELAQERSLQVPRSGDGGGGRVRIEKMSS EVVDSNPYSRLMALKRMGIVSDYEKIRTFAVAIVGVGGVGSVTAEMLTRCGIGKLLLFDYDKVEL ANMNRLFFQPHQAGLSKVQAAEHTLRNINPDVLFEVHNYNITTVENFQHFMDRISNGGLEEGKPV DLVLSCVDNFEARMTINTACNELGQTWMESGVSENAVSGHIQLIIPGESACFACAPPLVVAANID EKTLKREGVCAASLPTTMGVVAGILVQNVLKFLLNFGTVSFYLGYNAMQDFFPTMSMKPNPQCDD RNCRKQQEEYKKKVAALPKQEVIQEEEEIIHEDNEWGIELVSEVSEEELKNFSGPVPDLPEGITV AYTIPKKQEDSVTELTVEDSGESLEDLMAKMKNM*Background 'UBA5 is a member of the ubiquitin-activating E1 family and UBA5 subfamily. Ubiquitin and ubiquitin-like proteins are recognized as covalently conjugated to various cellular substrates by a three-step enzymatic pathway. The ubiquitin-activating enzyme (E1) has a vital role in the first step of ubiquitination pathway to activate ubiquitin or ubiquitin-like proteins. UBA5 activates ubiquitin-fold modifier 1, a ubiquitin-like post-translational modifier protein, via the formation of a high-energy thioester bond. UBA5 is located primarily in cytoplasm, while it generally localizes to the nucleus in presence of SUMO2.'