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CG48
Recombinant Human Ubiquitin-like protein Nedd8/NEDD8
10ug
160
144
现货
国产
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CG48
Recombinant Human Ubiquitin-like protein Nedd8/NEDD8
50ug
360
324
现货
国产
-
CG48
Recombinant Human Ubiquitin-like protein Nedd8/NEDD8
500ug
2000
1800
现货
国产
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CG48
Recombinant Human Ubiquitin-like protein Nedd8/NEDD8
1mg
3200
2880
现货
国产
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Catalog# CG48 Source E.coli Description Recombinant Human Neural precursor cell expressed developmentally down-regulated protein 8/NEDD8 is produced with our E. coli expression system. The target protein is expressed with sequence (Met1-Gly76) of Human NEDD8 fused with a 6His tag at the N-terminus. Names Neural precursor cell expressed developmentally down-regulated protein 8, NEDD8, Neddylin, Ubiquitin-like protein Nedd8, Accession # Q15843 Formulation Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence MGHHHHHHGSLQDSEVNQEAKPEVKPEVKPETHINLKVSDGSSEIFFKIKKTTPLRRLMEAFAKR QGKEMDSLRFLYDGIRIQADQAPEDLDMEDNDIIEAHREQIGGMLIKVKTLTGKEIEIDIEPTDK VERIKERVEEKEGIPPQQQRLIYSGKQMNDEKTAADYKILGGSVLHLVLALRGGBackground Human NEDD8 shares 60% amino acid sequence identity to ubiquitin. The only known substrates of NEDD8 modification are the cullin subunits of SCF ubiquitin E3 ligases. The NEDDylation of cullins is critical for the recruitment of E2 to the ligase complex, thus facilitating ubiquitin conjugation. NEDD8 modification has therefore been implicated in cell cycle progression and cytoskeletal regulation.