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C547
Recombinant Human Vanin-1/Pantetheinase/VNN1
10ug
840
756
现货
国产
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C547
Recombinant Human Vanin-1/Pantetheinase/VNN1
50ug
2520
2268
现货
国产
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C547
Recombinant Human Vanin-1/Pantetheinase/VNN1
500ug
12320
11088
现货
国产
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C547
Recombinant Human Vanin-1/Pantetheinase/VNN1
1mg
17600
15840
现货
国产
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Catalog# C547 Source HEK293 Description Recombinant Human Vascular Non-Inflammatory Molecule 1/Vanin-1 produced by transfected human cells is a secreted protein with sequence (Gln22-Ser490) of Human VNN1 fused with a polyhistidine tag at the C-terminus. Names Pantetheinase, Pantetheine Hydrolase, Tiff66, Vascular Non-Inflammatory Molecule 1, Vanin-1, VNN1 Accession # O95497 Formulation Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/μg (1 IEU/μg). Amino Acid Sequence QDTFTAAVYEHAAILPNATLTPVSREEALALMNRNLDILEGAITSAADQGAHIIVTPEDAIYGWN FNRDSLYPYLEDIPNPEVNWIPCNNRNRFGQTPVQERLSCLAKNNSIYVVANIGDKKPCDTSDPQ CPPDGRYQYNTDVVFDSQGKLVARYHKQNLFMGENQFNVPKEPEIVTFNTTFGSFGIFTCFDILF HDPAVTLVKDFHVDTIVFPTAWMNVLPHLSAVEFHSAWAMGMRVNFLASNIHYPSKKMTGSGIYA PNSSRAFHYDMKTEEGKLLLSQLDSHPSHSAVVNWTSYASSIEALSSGNKEFKGTVFFDEFTFVK LTGVAGNYTVCQKDLCCHLSYKMSENIPNEVYALGAFDGLHTVEGRYYLQICTLLKCKTTNLNTC GDSAETASTRFEMFSLSGTFGTQYVFPEVLLSENQLAPGEFQVSTDGRLFSLKPTSGPVLTVTLF GRLYEKDWASNASSVDHHHHHHBackground Vanin-1 is a cell membrane protein which contains one CN hydrolase domain and belongs to the CN hydrolase family and BTD/VNN subfamily. Vanin-1 is also a member of the Vanin family of proteins, which share extensive sequence similarity with each other, and also with biotinidase. The family includes secreted and membrane-associated proteins, a few of which have been reported to participate in hematopoietic cell trafficking. Vanin-1 is widely expressed with higher expression in spleen, kidney and blood and overexpressed in lesional psoriatic skin. No biotinidase activity has been demonstrated for any of the vanin proteins; however, they possess pantetheinase activity, which may play a role in oxidative-stress response. Vanin-1 is an epithelial pantetheinase that provides cysteamine to tissue and regulates response to stress. Vanin-1 is expressed by enterocytes, and its absence limits intestinal epithelial cell production of proinflammatory signals. Vanin-1 regulates late adhesion steps of thymus homing under physiological, noninflammatory conditions. The early impact of vanin-1 deficiency on tumor induction was directly correlated to the amount of inflammation and subsequent epithelial proliferation rather than cell death rate. Vanin-1 molecule was shown to be involved in the control of thymus reconstitution following sub-lethal irradiation.
References Spry C,et al.Pantothenamides Are Potent, On-Target Inhibitors of Plasmodium falciparum Growth When Serum Pantetheinase Is Inactivated
PMID:23405100
http://www.ncbi.nlm.nih.gov/pubmed/23405100