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CD73
Recombinant Human Vascular endothelial growth factor A/VEGF-A164
10ug
1400
1260
现货
国产
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CD73
Recombinant Human Vascular endothelial growth factor A/VEGF-A164
50ug
3600
3240
现货
国产
-
CD73
Recombinant Human Vascular endothelial growth factor A/VEGF-A164
500ug
12320
11088
现货
国产
-
CD73
Recombinant Human Vascular endothelial growth factor A/VEGF-A164
1mg
17600
15840
现货
国产
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Catalog# CD73 Source P. pastoris Description Recombinant Mouse VEGF-A164 is produced by our Pichia pastoris expression system. The target protein is expressed with sequence (Ala27-Arg190) of Mouse VEGF-1. Names Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGFA, VEGFA164, VEGF164 Accession # Q00731-2 Formulation Lyophilized from a 0.2 μm filtered solution of PBS，pH 7.4 Shipping The product is shipped at ambient temperature. Reconstitution Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.Storage Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.Purity Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE. Endotoxin Less than 0.1 ng/µg (1 IEU/µg). Amino Acid Sequence APTTEGEQKSHEVIKFMDVYQRSYCRPIETLVDIFQEYPDEIEYIFKPSCVPLMRCAGCCNDEAL ECVPTSESNITMQIMRIKPHQSQHIGEMSFLQHSRCECRPKKDRTKPENHCEPCSERRKHLFVQD PQTCKCSCKNTDSRCKARQLELNERTCRCDKPRRBackground Vascular endothelial growth factor (VEGF or VEGFA), also known as vascular permeability factor (VPF), is a potent mediator of both angiogenesis and vasculogenesis in the fetus and adult . It is a member of the PDGF family that is characterized by a cystine knot structure formed by eight conserved cysteine residues . Alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids (aa) have been identified in humans, with 120, 164 and 188 aa isoforms found in mouse . VEGF binds the type I transmembrane receptor tyrosine kinases VEGF R1 (also called Flt1) and VEGF R2 (Flk/KDR) on endothelial cells .Although affinity is highest for binding to VEGF R1, VEGF R2 appears to be the primary mediator of VEGF angiogenic activity . Human VEGF165 binds the semaphorin receptor, Neuropilin1 and promotes complex formation with VEGF R2 . VEGF is required during embryogenesis to regulate the proliferation, migration, and survival of endothelial cells . In adults, VEGF functions mainly in wound healing and the female reproductive cycle . Pathologically, it is involved in tumor angiogenesis and vascular leakage . Circulating VEGF levels correlate with disease activity in autoimmune diseases such as rheumatoid arthritis, multiple sclerosis and systemic lupus erythematosus. VEGF is induced by hypoxia and cytokines such as IL1, IL6, IL8, oncostatin M and TNFα .