||Recombinant Human ZW10 Interactor/ZWINT is produced by our E. coli expression system. The target protein is expressed with sequence (Met1-Pro277) of Human ZWINT fused with a His tag at the N-terminus.
||ZW10 Interactor, ZW10-Interacting Protein 1, Zwint-1, ZWINT
||Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2
||The product is shipped at ambient temperature.
||Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 1X PBS.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
||Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
||Greater than 95% as determined by reducing SDS-PAGE.
||Less than 0.1 ng/μg (1 IEU/μg).
|Amino Acid Sequence
MGSSHHHHHHSSGLVPRGSHMEAAETEAEAAALEVLAEVAGILEPVGLQEEAELPAKILVEFVVD SQKKDKLLCSQLQVADFLQNILAQEDTAKGLDPLASEDTSRQKAIAAKEQWKELKATYREHVEAI KIGLTKALTQMEEAQRKRTQLREAFEQLQAKKQMAMEKRRAVQNQWQLQQEKHLQHLAEVSAEVR ERKTGTQQELDGVFQKLGNLKQQAEQERDKLQRYQTFLQLLYTLQGKLLFPEAEAEAENLPDDKP QQPTRPQEQSTGDTMGRDPGVSFKAVGLQPAGDVNLP
||ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interphase cells. ZWINT interacts ZW10, MIS12 and NDC80 subunit of the NDC80 complex specifically during mitosis. ZWINT is a part of the MIS12 complex which is required for kinetochore formation and spindle checkpoint activity. In addition, ZWINT is required to target ZW10 to the kinetochore at prometaphase.